Journal: Pharmaceutical Biology
Article Title: Apigenin attenuates the atherosclerotic lesions through enhancing selective autophagy/lipophagy and promoting RCT process
doi: 10.1080/13880209.2025.2509020
Figure Lengend Snippet: Apigenin activated and promoted autophagy in ApoE −/− mice. (A) Western blot analysis of ULK1, UVRAG and beclin-1 were shown in left, and graph on the right represented the quantification analysis of autophagy proteins normalized by β-actin ( n = 3). (B) Western blot analysis of ATG3, ATG5 and LC3 were shown in left, and graph on the right represented the quantification analysis of autophagy proteins normalized by α-tubulin ( n = 3). (C) The mRNA expression levels of UVRAG, LC3B, Beclin-1 and ATG14 were examined by RT-qPCR ( n = 10). # p < 0.05, ## p < 0.01 vs NC group; * p < 0.05 and ** p < 0.01.
Article Snippet: The following primary antibodies were used: β-actin (ab8226, 1:2000, Abcam, RRID: AB_3696461, UniProt ID: P60709 ), α-tubulin (ab7291, 1:5000, Abcam, RRID: AB_3696470, UniProt ID: P68366 ), ATP-binding cassette transporter 5 (ABCG5) (27722-1-AP, 1:1000, Proteintech, RRID: AB_2880952, UniProt ID: Q9H222 ), Beclin-1 (66665-1-Ig, 1:2000, Proteintech, RRID: AB_2882020, UniProt ID: Q14457 ), unc-51-like autophagy-activating kinase 1 (ULK1) (20986-1-AP, 1:1000, Proteintech, RRID: AB_2878783, UniProt ID: O75385 ), UVRAG (19571-1-AP, 1:1000, Proteintech, RRID: AB_10640523, UniProt ID: Q9P2Y5 ), LC3 (18725-1-AP, 1:2000, Proteintech, RRID: AB_2137745, UniProt ID: Q9GZQ8 ), autophagy related protein 3 (ATG3) (11262-2-AP, 1:1000, Proteintech, RRID: AB_2059234, UniProt ID: Q9NT62 ), autophagy related protein 5 (ATG5) (10181-2-AP, 1:2000, Proteintech, RRID: AB_2062045, UniProt ID: Q9H1Y0 ), scavenger receptor class B type I (SR-BI) (ab217318, 1:2000, Abcam, RRID: AB_3696478, UniProt ID: Q8WTV0 ).
Techniques: Western Blot, Expressing, Quantitative RT-PCR
